ABSTRACT
Synthetic LHRHa or its superactive analogues stimulate GTH release. It shows an overlap in the biological activity of LHRHa and GnRH in many teleost species. The aim of the present study was to investigate the effects of LHRIIa[2]. Metocloprarnide and Chlorpromazine on HPG axis and plasma levels of GHT II. Luteinizing Hormone Releasing Hormone Analogue 2 [LHRHa[2]] [3 micro g/kg] with dopamine antagonis's [Metoclopramide. [5 mg/kg] and Chlorpromazine [10 mg/kg]] were injected via I.M to 64 female Bream [Abramis brama orientalis] [0.71371 +/- 0.026 kg, BW] through combined and solilary teeatments: Positive Control [saline]. Metoclopramide [5 mg/kg]. Chlorpromazine [10 mg/kg]. LHRHa[2] [3 micro g/kg]. Metoclopramide [5 mg/kg] + LHRHa[2] [3 micro g/kg], Chlorpromazine [10 mg/kg]+LHRHa[2] [3 micro g/kg]. Metoclopramide [5 mg/kg] + Chlorpromazine [10 mg/kg]+LHRHa[2] [3 micro g/kg] and Negative Control [intact]. Fishes were examined through primary bleeding. injection and secondary bleeding. The time interval between two bleeding step was 5h. The GTH 11 measurement was conducted on the basis of RIA. Results indicate that while LHRHa, and Metocloptamide could significantly increase the level of GTH II [p<0.05]. Chlorpromazine did not showed any significant impact on the increase of GTH II [p> 0.05]. Meanwhile, compared to the complex treatments the individual treatments have had relatively significant effects [p< 0.05]. All compounds showed a positive stimulation effect on the HPG axis and GTH 11 level in plasma